LNCRNA H19 Is Increased In Hepatocellular Carcinoma With Underlying Hcv-Related Cirrhosis
AASLD LiverLearning®. García-Valdecasas M. Nov 14, 2016; 144848
Topic: Experimental Hepatocarcinogenesis
Mrs. Marta García-Valdecasas
Mrs. Marta García-Valdecasas
Login now to access Regular content available to all registered users.

AASLD Members enjoy free LiverLearning® Premium Access. Registrants of The Liver Meeting® receive LiverLearning® Premium Access for one year from the first day of the meeting.

You may also access AASLD content "anytime, anywhere" with the FREE AASLD LiverLearning® App for iOS and Android.

Discussion Forum (0)
Rate & Comment (0)

TITLE: LNCRNA H19 Is Increased In Hepatocellular Carcinoma With Underlying Hcv-Related Cirrhosis

No sponsors

INTRODUCTION: HCV infection induces cirrhosis development, increasing hepatocellular carcinoma (HCC) risk. The non-coding RNA deregulation can result in cancer. We aimed to analyze the expression of lncRNA and miRNAin both HCV-cirrhotic and HCV-HCC patients.
METHODS: Liver biopsies from cirrhotic patients without (n=7) and with HCC (n=7), infected with HCV genotype 1 (n=10) and 4 (n=4), were paired in two groups. In both groups, liver biopsies were taken from non-tumour areas. Total RNA were isolated by mirVana™ kit (ThermoFisher). RNA were pooled in the 2 groups and 84 lncRNAs were compared by RT2 IncRNA PCR Array Cancer(Qiagen) whereas 352miRNA expression were compare withEPIK™ Cancer miRNA(Bioline). Significant lncRNAs were proved in each patient by qPCR.
RESULTS: From 84 lncRNAs studied, five of themshowed a higher expression in HCC patients (H19: 2.93, PCAT1: 3.12, AFAP1-AS1: 4.72, CCAT2: 3.07, PTENP1: 3.10 fold induction). Individual expression of these lncRNAs were measured, but only H19 was found to be significantly increased (p=0.013) (Fig. 1).
miRNA expression was also compared between cirrhotic and HCC patients. We found eleven miRNA significantly altered: a) Six of them were decreased (miR-19b-3p: 4.15, miR-199a-5p: 3.82, miR382-5p: 3.06, miR:99a-5p: 3.35, miR200b-3p: 2.77, miR-192-5p: 2.57 fold inhibition); b) Five were increased (miR-216b-5p: 3.65, miR-1290: 3.57, miR-1246: 2.80, miR-331-3p: 2.82, miR-511-5p: 2.48 fold induction).
CONCLUSION: lncRNA H19 was increased in cirrhotic patients showing HCV-related HCC in comparison with those without it. Thus, H19 could be an interesting lncRNA to predict HCC.

Code of conduct/disclaimer available in General Terms & Conditions
Anonymous User Privacy Preferences

Strictly Necessary Cookies (Always Active)

MULTILEARNING platforms and tools hereinafter referred as “MLG SOFTWARE” are provided to you as pure educational platforms/services requiring cookies to operate. In the case of the MLG SOFTWARE, cookies are essential for the Platform to function properly for the provision of education. If these cookies are disabled, a large subset of the functionality provided by the Platform will either be unavailable or cease to work as expected. The MLG SOFTWARE do not capture non-essential activities such as menu items and listings you click on or pages viewed.

Performance Cookies

Performance cookies are used to analyse how visitors use a website in order to provide a better user experience.

Google Analytics is used for user behavior tracking/reporting. Google Analytics works in parallel and independently from MLG’s features. Google Analytics relies on cookies and these cookies can be used by Google to track users across different platforms/services.

Save Settings