ABSTRACT FINAL ID:
LNCRNA H19 Is Increased In Hepatocellular Carcinoma With Underlying Hcv-Related CirrhosisSPONSORSHIP - THIS STUDY WAS SPONSORED BY: (IF THIS ABSTRACT WAS NOT SPONSORED PLEASE INDICATE):
No sponsorsABSTRACT BODY:
INTRODUCTION: HCV infection induces cirrhosis development, increasing hepatocellular carcinoma (HCC) risk. The non-coding RNA deregulation can result in cancer. We aimed to analyze the expression of lncRNA and miRNAin both HCV-cirrhotic and HCV-HCC patients.
METHODS: Liver biopsies from cirrhotic patients without (n=7) and with HCC (n=7), infected with HCV genotype 1 (n=10) and 4 (n=4), were paired in two groups. In both groups, liver biopsies were taken from non-tumour areas. Total RNA were isolated by mir
Vana™ kit (ThermoFisher). RNA were pooled in the 2 groups and 84 lncRNAs were compared by RT2 IncRNA PCR Array Cancer(Qiagen) whereas 352miRNA expression were compare withEPIK™ Cancer miRNA(Bioline). Significant lncRNAs were proved in each patient by qPCR.
RESULTS: From 84 lncRNAs studied, five of themshowed a higher expression in HCC patients (H19: 2.93, PCAT1: 3.12, AFAP1-AS1: 4.72, CCAT2: 3.07, PTENP1: 3.10 fold induction). Individual expression of these lncRNAs were measured, but only H19 was found to be significantly increased (p=0.013) (Fig. 1).
miRNA expression was also compared between cirrhotic and HCC patients. We found eleven miRNA significantly altered: a) Six of them were decreased (miR-19b-3p: 4.15, miR-199a-5p: 3.82, miR382-5p: 3.06, miR:99a-5p: 3.35, miR200b-3p: 2.77, miR-192-5p: 2.57 fold inhibition); b) Five were increased (miR-216b-5p: 3.65, miR-1290: 3.57, miR-1246: 2.80, miR-331-3p: 2.82, miR-511-5p: 2.48 fold induction).
CONCLUSION: lncRNA H19 was increased in cirrhotic patients showing HCV-related HCC in comparison with those without it. Thus, H19 could be an interesting lncRNA to predict HCC.